Table 1 Design of IVT Interferon alpha mRNA variants (IFN-α var1 – IFN-α var5).
IVT mRNA | 5′ and 3′ UTR | Sequence variation (%) | GC content (CDS) | Codon optimization |
|---|---|---|---|---|
IFN α | human IFN α | — | 48.7 | − |
IFN α var1 | modified | — | 48.7 | − |
IFN α var2 | modified | 21.5 | 49.9 | + |
IFN α var3 | modified | 21 | 56.8 | + |
IFN α var4 | modified | 23 | 40.4 | + |
IFN α var 5 | modified | 21 | 60 | + |
