Figure 6 | Scientific Reports

Figure 6

From: Isoform-specific hyperactivation of calpain-2 occurs presymptomatically at the synapse in Alzheimer’s disease mice and correlates with memory deficits in human subjects

Figure 6

Activity levels of synaptic calpain-2 are upregulated pre-symptomatically in APP/PS1 mice from 1 month onwards. Immunoprecipitation of calpain-2 was employed to assay isoform-specific activity of calpain-2 in synaptosomal samples isolated from brain cortices of APP/PS1 mice. Increased activity of calpain-2 was observed from 1 month of age (A), persisting up to 3 months (B) and later up to 10 months (C). Isoform specific activity of calpain-1 assayed after immunoprecipitation of calpain-1 (See Supplementary Fig. 1) in cortical synaptosomes of APP/PS1 mice was unchanged at 1 month of age (D). Each of the individual panels show both the representative activity curves and the cumulative data. Values are mean ± SD (n = 7 mice) and * denotes values significantly different from corresponding controls (p < 0.05; Mann Whitney test). See also Supplementary Fig. 1.

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