Figure 5 | Scientific Reports

Figure 5

From: The splicing factor SRSF3 is functionally connected to the nuclear RNA exosome for intronless mRNA decay

Figure 5

Intronless mRNAs do not hyperaccumulate in the nucleus upon co-depletion of the RNA exosome and the SRSF3 protein. (a) Immunoblot: Western blotting analysis showing protein depletion upon the indicated siRNA treatments. Anti-Tubulin antibody was used as a loading control. * indicates non-specific bands. (b) Nuclear mRNA accumulation: RT-qPCR analysis of nuclear RNAs from HeLa cells transfected with the indicated siRNAs together with the Renilla luciferase and BDLF1 intronless constructs. Data are displayed as mean values normalized to the control siRNA and GAPDH mRNA as an internal control. Error bars represent the s.d. from three independent experiments (n = 3).

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