Figure 2

Heterotrophic carbon and nitrogen assimilation of coral gastrodermis. Correlated TEM and NanoSIMS images of the distribution of prey 13C (A,E) and 15N (C,F) in the surface body wall gastrodermis 6 h after ingestion of labelled Artemia salina began. Subcellular features abbreviated as in Fig. 1, with OE indicating the surface body wall epidermis and MG the mesoglea. Multiple gastrodermal 13C and 15N hotspots were observed (coloured arrows, detailed in Figs. 3, 4). Hotspots of 13C in the symbionts corresponded to starch (<). Colours in NanoSIMS maps represent enrichment relative to an unlabelled tissue as atomic percent excess (APE) (note log scale in C,F). Asterisks indicate host nucleoli. Scale bars are 2 µm.