Figure 3

Types of isotopic hotspots in the gastrodermis from heterotrophy. Correlated TEM and NanoSIMS images for 13C (A,B) and 15N (A,C) highlighting three types of isotopic hotspots (coloured arrows; see text) with TEM ultrastructure and partial 15N-signal overlay (D–F). Note the presence of a membrane around the hotspot in D (>). Colours in (B,C) display enrichment relative to an unlabelled tissue as atom percent excess (APE). Scale bars are 1 µm (A–C, E,F) and 750 nm (D).