Figure 4 | Scientific Reports

Figure 4

From: Temperature and feeding induce tissue level changes in autotrophic and heterotrophic nutrient allocation in the coral symbiosis – A NanoSIMS study

Figure 4

Characteristics of isotopic hotspots in the gastrodermis from heterotrophy. Multiple high-resolution (15 × 15 µm) TEM and NanoSIMS images (A–C) were analysed for size (D) and individual enrichment (E) of the three types of isotopic hotspots in the gastrodermis. CSS: cup-shaped structures (blue arrows), PAT: electron-lucent patches (green), VES: vesicles (pink). Canonical correlation analysis of these characteristics revealed a distinct CSS cluster with overlapping PAT and VES clusters (F). Colours in (B) and (C) display enrichment relative to an unlabelled tissue as atom percent excess (APE). Scale bars are 1 µm.

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