Figure 1

An overview of individual tumor spheroid isolation from malignant ascites. (A) A malignant ascites was collected during a primary debulking surgery. Tumor spheroids in the malignant ascites were purified, fixed and prepared on a discharging layer (Indium Tin Oxide (ITO), 100 nm in thickness)-coated glass slide. (B) The laser isolation technique was used to isolate individual tumor spheroids. This technique utilizes an IR pulsed laser, which vaporizes the discharging layer on the glass slide. Using this technique, ten individual tumor spheroids were isolated from the slide. The isolated cells underwent WGA and sequencing. (C) The images before and after isolation demonstrate that the targeted tumor spheroids in the malignant ascites were specifically isolated without disturbing the neighboring cells. The scale bars represent 100 μm.