Figure 5
Analysis of the allele frequency to infer the cellular composition of each sample. The VAF distribution was plotted for each sample from the (A) Primary site and (B,C) Ascites. The mutations were categorized into common, primary-only or ascites-only mutations. Common mutations were somatic SNVs, which were detected in both the Primary clone and Ascites clones, and primary-only and ascites-only mutations, which were shared somatic SNVs detected only in the Primary clone and Ascites clones, respectively. The results showed that most of the VAF distributions from Ascites clone 2 were located at a much lower range than those from the Primary clone and Ascites clone 1. This suggests that the tumor spheroids in Ascites clone 2 had a large proportion of normal cells in each tumor spheroid.
