Figure 6
Constructing phylogenetic trees and inferred evolutionary history of the tumor. Phylogenetic trees were constructed using both the (A) CNA profiles and (B) SNV profiles. The two trees presented similar topologies and indicated that the Primary clone and Ascites clone 1 were derived from one ancestral clone at the early stage of cancer development. In addition, the phylogenic trees indicate early monoclonal and unidirectional seeding of tumor spheroids in Supplementary Fig. S3 malignant ascites and no additional clonal seeding from the primary site in this patient. (C) Based on the sequencing data from the primary tissue samples and the tumor spheroids from the ascites, the evolutionary trajectory was inferred. The tumor was initiated at the right ovary to generate the ancestral clone. With the further accumulation of mutations, the ancestral clone evolved into two subclones, the first of which was found in the right ovary and metastasized to the left ovary. The second subclone shed into the ascites TME and became extinct or dominated by the first subclone in the right ovary. Eventually, the Ascites subclone moved to the peritoneal cavity.
