Figure 3 | Scientific Reports

Figure 3

From: Histone deacetylase-mediated regulation of the antimicrobial peptide hBD2 differs in intestinal cell lines and cultured tissue

Figure 3

Interleukin 1β (IL1β)-mediated human β-defensin 2 (hBD2) induction is enhanced by histone deacetylase (HDAC) inhibition in HCT116 cells. (A) Induction level of hBD2 mRNA in HCT116 cells by IL1β (10 ng/ml) after 18 hrs of stimulation. Fold change in hBD2 (B) or IL8 (C) induction are shown compared to IL1β stimulation alone when co-treated with either SAHA (5 µM), MS-275 (2 µM), or SB (3 mM). Inhibitor treatment and stimulation were done exactly as for CaCo-2/TC7 cells. Depicted are results of at least three independent experiments carried out in biological triplicates. *p < 0.05, **p < 0.01, evaluated by Mann-Whitney u test.

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