Figure 5 | Scientific Reports

Figure 5

From: Histone deacetylase-mediated regulation of the antimicrobial peptide hBD2 differs in intestinal cell lines and cultured tissue

Figure 5

Inhibition of histone deacetylases (HDACs) hinders human β-defensin 2 (hBD2) induction in human colonic biopsies. HBD2 mRNA expression in cultured human colonic biopsies of (A) 13 healthy controls and (B) four colorectal tumor specimens in response to EcN, alone or together with either SAHA (5 µM), MS-275 (5 µM), or SB (3 mM). Inhibitor treatment started 2 hrs prior to the stimulation with EcN, which took then place in parallel to HDAC inhibition for another 18 hrs. (C) Hematoxylin and eosin staining of an uncultured (0 h) and medium cultured (20 h) healthy control biopsy. (D) HBD2 mRNA expression in colonic biopsies of 29 patients with Crohn’s Disease (CD) or 14 patients with ulcerative colitis (UC) (E). (F) Direct comparison of the hBD2 fold inductions in both disease entities CD or UC after EcN stimulation. Shown are relative fold changes compared to medium treatment alone according to 10 ng total RNA normalized to βactin expression. *p < 0.05, **p < 0.01, ***p < 0.001, ****p < 0.0001 evaluated by Mann-Whitney u test.

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