Figure 3
From: Endogenous Galectin-9 Suppresses Apoptosis in Human Rheumatoid Arthritis Synovial Fibroblasts
RA synovial fibroblasts were stimulated with (A) TLR agonists, TNFα, IL-1β, bLP, poly(I:C), LPS and (B) pro-inflammatory cytokines –IL-1β, IL-4, IL-6, IL-17, IFNγ, TNFα and TGFβ. mRNA was extracted and fold-changes were plotted. (C) The increase in Gal9 expression following IFNγ stimulation was confirmed at the protein level by Western blotting. Long (39 KDa) and short (35 KDa) isoforms of Gal9 are shown. The short isoform was induced by IFNγ stimulation whereas the long isoform is constitutively expressed. *p < 0.05, **p < 0.01. Full uncropped versions of the blots are shown in the supplementary data.
