Table 1 LOD and amplification reproducibility of the primer and probe on TAC.
Targeted pathogens | Linearity R2 efficiency (%) | Limit of detection (LOD) (CFU/mL) | Ct at LOD | Reproducibility of amplification efficiency CV | |
|---|---|---|---|---|---|
Inter-assay | Intra-assay | ||||
S. aureus | 0.999 (91.136) | 101 | 35.94 | 1.48% | 0.28% |
S. epidermidis | 1.000 (93.478) | 101 | 38.48 | 0.73% | 1.69% |
S. hominis | 0.998 (90.83) | 100 | 39.08 | 3.37% | 6.33% |
S. haemolyticus | 0.970 (95.199) | 100 | 35.11 | 1.99% | 9.63% |
K. oxytoca | 0.985 (85.925) | 101 | 37.51 | 1.47% | 3.08% |
E. faecalis | 1.000 (95.176) | 101 | 37.96 | 1.22% | 1.45% |
E. faecium | 0.924 (98.241) | 101 | 35.75 | 1.20% | 5.00% |
S. pneumoniae | 0.999 (92.845) | 101 | 34.59 | 1.47% | 0.83% |
S. agalactiae | 0.999 (93.03) | 101 | 37.91 | 0.60% | 8.20% |
S. pyogenes | 0.992 (95.359) | 100 | 36.80 | 1.31% | 11.58% |
C. perfringens | 0.992 (89.379) | 102 | 37.85 | 0.46% | 1.58% |
S. marcescens | 0.996 (96.078) | 101 | 38.19 | 1.84% | 5.66% |
L. monocytogenes | 0.999 (102.677) | 101 | 36.06 | 1.90% | 2.75% |
E. coli | 0.993 (102.611) | 101 | 36.29 | 2.74% | 7.50% |
K. pneumoniae | 1.000 (91.873) | 101 | 37.94 | 0.94% | 0.40% |
Ab | 0.979 (102.292) | 101 | 37.11 | 0.38% | 6.37% |
S. maltophilia | 0.996 (91.848) | 100 | 37.89 | 1.58% | 2.08% |
P. aeruginosa | 0.992 (92.918) | 102 | 36.28 | 1.31% | 1.68% |
A. xylosoxidans | 0.991 (100.652) | 100 | 38.04 | 1.33% | 0.35% |
B. cepacia | 0.999 (90.976) | 101 | 38.14 | 1.88% | 2.65% |
N. meningitides | 0.998 (95.360) | 102 | 36.92 | 0.40% | 0.72% |
H. influenzae | 0.999 (92.914) | 101 | 36.42 | 0.67% | 1.70% |
C. neoformans | 0.996 (93.441) | 101 | 38.26 | 1.30% | 2.49% |
C. glabrata | 0.999 (92.075) | 101 | 37.72 | 1.17% | 1.52% |
C. tropicalis | 0.996 (96.290) | 101 | 38.99 | 0.41% | 7.62% |
C. albicans | 0.995 (91.746) | 101 | 34.90 | 4.49% | 3.74% |
C. parapsilosis | 0.999 (93.193) | 100 | 34.17 | 0.59% | 0.76% |
