Figure 1
From: Separation and Loss of Centrioles From Primordidal Germ Cells To Mature Oocytes In The Mouse
GFP CETN2 centriole detection in isolated mouse primordial germ cells and fetal oogonia. (A,B) Isolated primordial germ cells (PGCs) from an e11.5 dpc female gonad. (A) Upper mitotic cell (blue, DNA) shows the presence of the germ cell marker SSEA-1 (red) at the cell periphery, along with two pairs of GFP CETN2 foci (inset) on opposite sides of the condensed prometaphase chromosomes (green, arrowheads). The lower somatic interphase gonadal cell lacks SSEA-1, with a single pair of GFP CETN2 focus (inset) adjacent to the nucleus (green, arrowheads). All GFP CETN2 centrioles are associated with microtubules, either at the center of microtubule asters at each nascent spindle pole (B: upper cell, red) or assembled on the nuclear surface connected to the more robust cortical microtubule interphase network (B: lower cell, red). (C,D) A divided e11.5 dpc primordial germ cell with SSEA-1 at the cell periphery of both daughter cells (C: red) and expressing GFP CETN2 centrioles (C: green, arrowheads) adjacent to the nuclei (C: blue, DNA). Microtubule assembly is observed only from the top daughter cell (D: red). (E,F) Early mouse oogonium from an e14.5 dpc gonad with cortical SSEA-1 (E: red) and GFP CETN2 centriole pairs (E: green, arrowheads; blue, DNA). Cortical microtubule bundles are pronounced at the site of the GFP CETN2 centriole pair (F: red, microtubules, arrowheads). (G,H) Cells isolated from the sex cord of an e18.5 dpc fetal gonad, showing a single oogonial cell labeled with SSEA-1 (G: red, arrow) with a pair of GFP CETN2 centrioles (G: green, double arrowheads) at the nucleus (blue, DNA) but without apparent microtubule assembly (H: red, double arrowheads). The remaining somatic interphase gonadal cells are SSEA-1-negative but express GFP CETN2 centriole pairs (G: green foci) at their nuclear surfaces near the site of interphase microtubule assembly (H: red, microtubules). Confocal images of GFP CETN2 (green) are counterstained with antibodies to SSEA-1 (A,C,E,G: red), microtubules (B,D,F,H: color assigned red) and DNA (blue). Insets in (A,C,E,G) details of GFP CETN2 centrioles. dpc: days post-coitus. Bars as marked.
