Figure 4
From: Separation and Loss of Centrioles From Primordidal Germ Cells To Mature Oocytes In The Mouse
Immunohistochemical analysis of oocytes in GFP CETN2 ovaries from preadolescent P4 and adult mice. (A1–A4) P4 primary oocyte showing GFP CETN2 doublets (A2: green, long arrows) in association with the PCM protein γ-tubulin (A3: red, arrowhead) adjacent to the GV nucleus (A1: blue, DNA). Image also shows a somatic follicular cell GFP CETN2 doublet (A2: short arrows) embedded in γ-tubulin (A3: red, double arrowheads). (A4) Triple overlays of image panels A1–A3. (B–B4) A pre-antral follicle with a primary oocyte from an adult ovary showing a single GFP CETN2 foci (B2: green, long arrow) within γ-tubulin (B3: red, arrowhead) at the GV nucleus (B1: blue, GV). A follicular cell (B1: blue, FC) with GFP CETN2 foci (B2: green, short arrow) in γ-tubulin (B3: red, double arrowhead) is also visible. (B4) Triple overlay of image panels B1-B3. (C–C4) An early antral follicle from an adult ovary showing a GV oocyte with a pair of GFP CETN2 foci (C2: green, long arrows) within γ-tubulin (C3: red, arrowheads) at the nucleus (C1: blue, GV) and a surrounding follicular cell with GFP CETN2 centrioles (C2: green, short arrows) and γ-tubulin (C3: red, double arrowheads). (C4) Triple overlays of C1-C3 image panels. All images are 7-µm sections through ovaries taken from females expressing GFP CETN2 and counterstained with γ-tubulin (red) and DNA (blue). Boxes in A, B, and C differential interference contrast (DIC) images are areas enhanced in the fluorescent image panels. Bars = 10 µm.
