Figure 5
Effects of pressure elevation and neurosteroids. (A–H) Light micrographs of the middle part of the retina incubated with AlloP alone (A–C), 24SH alone (D–F), and combination with AlloP and 24SH (G–H) at 75 mmHg. (A–C) Administration of 1 μM AlloP (A) and 0.2 μM AlloP (B) substantially protected the retina from pressure-induced injury, whereas 0.1 μM AlloP (C) did not inhibit axonal swelling (arrowheads) completely. (D–F) Administration of 1 μM 24SH (D) and 0.2 μM 24SH (E) substantially protected the retina from pressure-induced injury, whereas 0.1 μM 24SH (F) did not inhibit axonal swelling (arrowheads) completely. (G) A combination of 0.1 μM AlloP and 0.1 μM 24SH provided complete protection. (H) A combination of 0.05 μM AlloP and 0.05 μM 24SH failed to inhibit pressure-induced axonal swelling (arrowheads). (A–H) are at the same magnification. Scale bars, 15 μm.
