Figure 8
LC-MS/MS analysis of AlloP and 24SH and summary of effects of neurosteroids, their inhibitors, APV, and pressure. (A) Measurement of AlloP (per wet retinal weight) in retinas using LC-MS/MS. AlloP levels significantly increased at 75 mmHg (*p < 0.05) compared to control pressure (10 mmHg). Administration of 50 μM APV blocked pressure-induced AlloP increase. (B) Measurement of 24SH (per wet retinal weight) in retinas using LC-MS/MS. 24SH levels significantly increased at 75 mmHg (*p < 0.05) compared to control pressure (10 mmHg). Administration of APV blocked pressure-induced 24SH increase. (C) AlloP levels significantly increased by administration of 0.1 μM 24SH compared to the retina incubated without 24SH (*p < 0.05). Administration of APV depressed 24SH-induced AlloP increase at 10 mmHg. Statistical significance is determined by Tukey’s multiple comparison test (*p < 0.05). (D) Summary of current findings along with a summary of our previous observation14,15,17. (D–A): Effects of normobaric and hyperbaric conditions on neurosteroidogenesis, axonal swelling and excitotoxicity. In the current work, we found 24SH raises AlloP levels to about 50 ng/g at 10 mmHg. Duta: Dutasteride, Voric: voriconazole. (D–B) Current findings about effects of various combinations of AlloP and 24SH on axonal swelling at 75 mmHg. (D–C) Current findings about effects of various combinations of AlloP and 24SH on excitotoxicity by voriconazole at 10 mmHg and by dutasteride at 75 mmHg. (E) The diagram presents a scheme depicting how 24SH and AlloP synthesis may interact via NMDA receptors in hyperbaric conditions.
