Figure 4 | Scientific Reports

Figure 4

From: One-step process for production of N-methylated amino acids from sugars and methylamine using recombinant Corynebacterium glutamicum as biocatalyst

Figure 4

Fed-batch cultivation with C. glutamicum NMeAla1 in minimal medium supplemented with potassium acetate and glucose as carbon and energy sources. A fermenter with an initial start volume of 4 L was used. First feed phase (potassium acetate) was coupled to the rDOS value. After 22 h the second feed phase was started by the initial addition of 162 mL of a glucose/MMA solution followed by a linear feed of 12.3 mL h−1. The biomass formation (black open squares), concentrations of NMeAla (red circles), l-alanine (blue squares), pyruvate (grey squares), acetate (green filled triangles) and glucose (green open triangles) were depicted. The volume of both feeds is shown as black lines. All depicted concentrations and the biomass formation was related to the initial volume.

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