Table 1 Bacterial trains and vectors used in this study.

WT

C. glutamicum wild type, ATCC13032

American Type Culture Collection

ELB-P

WT carrying deletions ΔaceE Δpqo ΔldhA ΔC-T ilvN ΔalaT ΔavtA

³¹

NMeAla1

WT carrying deletions ΔaceE Δpqo ΔldhA ΔC-T ilvN ΔalaT ΔavtA and vector pVWEx1-dpkA

This work

pVWEx1

Kan^R, C. glutamicum/E. coli shuttle vector (P_tac, lacI, pHM1519 oriV_C.g.)

⁸⁵

pEKEx3

Spec^R, C. glutamicum/E. coli shuttle vector (P_tac, lacI, pBL1 OriV_C.g.)

⁸⁶

pECXT99A

Tet^R, C. glutamicum/E. coli shuttle vector (P_trc, lacI, pGA1 OriV_C.g.)

⁸⁷

pVWEx1-dpkA

Kan^R, pVWEx1 overexpressing dpkA from P. putida KT2440 and change of start codon GTG to ATG

This work

pEKEx3-xylA_Xc-XylB_Cg

Spec^R, pEKEX3 overexpressing xylA from Xanthomonas campestris SCC1758 and xylB from C. glutamicum ATCC 13032

⁴⁵

pECXT99A-araBAD

Tet^R, pECXT99A overexpressing araBAD from E. coli MG1655

This work

pECXT99A-amyA

Tet^R, pECXT99A overexpressing amyA from Streptomyces griseus IMRU3570

⁴²