Figure 2

PGE2 enhanced lipogenic metabolism in hepatocytes. (A) TG level treated with PGE2 (10 nM) without and with L161, 982 (1 μM). (B) Cells treated with PGE2 and/or L161, 982 for 12 hours and then changed to tyrode solution. Cells were stained with NileRed (1 μM) for 30 minutes in tyrode solution in the incubator. Scale bars, 10 μm. (C) Quantification of the fluorescence intensity of NileRed. The data are shown as mean ± SEM with * for p < 0.05, ** for p < 0.01, *** for p < 0.001. The same experiments were repeated at least three times with similar results.