Figure 2
From: Microparticles from β-thalassaemia/HbE patients induce endothelial cell dysfunction
MPs induced coagulation protein expression. Unprimed and LPS-primed HUVECs were treated with 1 × 106 and 5 × 106 particles/mL MPs obtained from 7 normal subjects, 9 non-splenectomised β-thalassaemia/HbE patients (BE-NS) and 9 splenectomised β-thalassaemia/HbE patients (BE-S). The expression of (A) tissue factor (TF) and (B) von Willebrand factor (vWF) were determined by RT-qPCR. (C) TF activity in supernatant secreted by HUVECs. Unprimed and LPS-primed HUVECs were treated with 1 × 106 and 5 × 106 particles/mL MPs obtained from 3 normal subjects and 3 splenectomised β-thalassaemia/HbE patients (BE-S). aStatistically significant difference when compared with unprimed HUVECs at P < 0.05. bStatistically significant difference when compared with LPS-primed HUVECs at P < 0.05. *Statistically significant difference between groups at P < 0.05. Each experiment was duplicated.
