Figure 5
From: Microparticles from β-thalassaemia/HbE patients induce endothelial cell dysfunction
MPs stimulate monocyte-endothelial cell adhesion. Unprimed and LPS-primed HUVECs were treated with 1 × 106 and 5 × 106 particles/mL MPs obtained from 7 normal subjects, 9 non-splenectomised β-thalassaemia/HbE patients (BE-NS) and 9 splenectomised β-thalassaemia/HbE patients (BE-S). HUVECs were stained with Hoechst (blue), and BCECF-AM-labeled THP-1 cell lines (green). (A) The illustration was captured fluorograph at ×200 magnification by fluorescent microscopy. (B) The fluorescent intensity of the amount of adhered THP-1 cells was detected by fluorescent plate reader and calculated by normalised with untreated HUVECs. aStatistically significant difference when compared with unprimed HUVECs at P < 0.05. bStatistically significant difference when compared with LPS-primed HUVECs at P < 0.05. *Statistically significant difference between groups at P < 0.05. Each experiment was duplicated.
