Figure 5

Decreases in GABA_A receptor subunits and GAD mRNA levels in the brain of Reln^rl-Orl/+ mice. The (a) nucleus accumbens, (b) cerebellum, and (c) medial prefrontal cortex. The transcripts in WT and Reln^rl-Orl/+ mice were quantified by real-time RT-PCR using the 2^−ΔΔCT method, and normalized to the internal reference housekeeping gene GAPDH. Data represent the mean ± SEM (n = 4 for WT mice; n = 3 to 4 for Reln^rl-Orl/+ mice). *p < 0.05, significantly different from WT mice (two-way ANOVA).