Figure 2
From: EpCAM homo-oligomerization is not the basis for its role in cell-cell adhesion
SAXS analysis shows no evidence of oligomerization. (a) Scaled SAXS profiles of ngEpEX at different concentrations in the range from 0.5 mg/ml (17.5 µM) to 26.2 mg/ml (919.4 µM) presented and overlaid in the same plot. (b) MW and Rg values calculated from SAXS profiles. Dotted lines represent values calculated for dimer structure 57 kDa and 24 Å. Predicted area of tetramer values are depicted with a grey rectangle and the hypothetical tetramer values at 114 kDa and 54 Å are marked with black triangles. (c) Three orientations of EpEX dimer structure docked in the ab initio shape (grey envelope) reconstructed from the merged SAXS profile. Subunits in the dimer are depicted as cyan and magenta ribbons.
