Figure 3
From: Tissue-engineered 3D melanoma model with blood and lymphatic capillaries for drug development
Formation of melanoma spheroids using the hanging drop method. Spheroids formed by (A) Malme 3 M, (B) A375, (C) RPMI 7951, (D) SK-MEL 28, (E) WM983A, (F) WM983B cell lines after 14 days of culture in GravityTRAPTM plates. (Scale bars: 100 µm). (G) Hematoxylin and eosin staining of paraffin embedded spheroid section showing heterogenous composition and formation of compact spheroids by WM983A cells (Scale bar: 100 µm). (H) Tumor spheroids incorporated to skin tissues can easily be located (red arrows, Malme 3 M cells). Scale bar: 1 cm.
