Figure 5

Histopathologic and immunohistochemical findings of puppies infected with CAdV-1 and CDV. There is necrohaemorrhagic hepatitis associated several intranuclear inclusion bodies (arrows) within hepatocytes of a mixed-breed dog (A), that demonstrated positive immunoreactivity to antigens of CAdV-1 (B). There is negative immunoreactivity to antigens of CAdV-1 in the oedematous gallbladder (C) but positive immunolabelling for CAdV-1 antigens within hepatocytes of the liver of a mixed breed puppy (#7) with necrohaemorrhagic hepatitis (D). Observe positive immunoreactivity to antigens of CDV in the oedematous gallbladder of puppy #7 (E), and positive immunolabelling of CAdV-1 antigens in epithelial cells of the bronchiole and mixed glands of the lungs of a Chihuahua puppy (#10) with interstitial pneumonia and necrotizing bronchiolitis (F). There is severe hepatocellular degeneration of the liver (G) of a Yorkshire terrier puppy (#15) with positive immunolabelling for CAdV-1 within the bile duct epithelium (H). (A,G) Haematoxylin and Eosin stain; (B–F,H), Immunoperoxidase; Bar, (A) 20 µm; (B–H) 50 µm.