Figure 4
E-cadherin deletion leads to an increase of nuclear β-catenin localization in the P4 pancreas. (A–D) Immunofluorescence analysis of β-catenin (red), Epcam (green), and DAPI (blue) in the control (A,C) and Cdh1ΔPan/ΔPan (B,D) pancreas at P0 and P4. Dashed white bounding boxes indicate dimensions of the projected area for the individual panels to the right (A’–B”). (E,F) Immunofluorescence analysis of active β-catenin (red), and DAPI (green) in the control (E) and Cdh1ΔPan/ΔPan (F) pancreas at P4. White asterisks indicate dilated/cystic exocrine structures. White arrows indicate nuclear β-catenin. White scale bars = 100 μm. (G) qRT-PCR analysis of the Wnt targets Axin2, Myc, and Ccnd1. Histograms represent mean ± SEM of at least three independent determinations. For 2-tailed t-tests, *p < 0.05 compared to control.
