Figure 2
From: Mammillary body regulates state-dependent fear by alternating cortical oscillations
Activity of the mammillary body is repressed upon MK-801 administration. (a) Representative images of c-Fos immunostaining in the MB, CA1, DG and septum under the vehicle and the MK-801 (i.p., 0.1 mg/kg) conditions. Green, c-Fos. Blue, DAPI. Scale bar, 200 μm. (b) MK-801 reduced the expression of c-Fos in the MB, CA1 and DG, but not septum (n = 5, 4 mice, unpaired t test). (c) Left, schema showing injection of AAV8: hSyn-GCaMP6f and subsequent fiber implantation into the MB. Right bottom, one example image of virus expression in the MB. Green, GCaMP6f. Scale bar, 200 μm. (d) Top, representative traces of calcium transients in the MB before and after (30 min later) vehicle (left) or MK-801(right) injection. Bottom left: injection of vehicle had no effect on the basal activity in the MB. Bottom right: MK-801 reduced both the amplitude and the frequency of the calcium transients in the MB (n = 7 from 5 mice each, paired t test) (Each point represents a normalized average signal of 5 min. Time of injection: 0 min; baseline: - 5 to 0 min; vehicle/MK-801: 30 to 35 min). ns: P > 0.05, **P < 0.01. Veh., vehicle. MK, MK-801. Data presented as mean ± SEM.
