Figure 1
From: Sex differences in lipid metabolism are affected by presence of the gut microbiota
Sex-specific effects in overall ileal gene expression and related biological functions. (A) PCA was performed on IQR-based top 1,000 most variable genes in microarray analysis of male and female GF and Conv mice. (B) Significantly up- and down-regulated genes of males compared to females shown for GF and Conv mice. (C,D) Significantly up- and down-regulated genes of male compared to female (p ≤ 0.01) mice were compared between GF and Conv mice and are presented as Venn diagrams (based on intensity-based moderate t-statistics (IBMT) regularised t-test p ≤ 0.01). (E,F) IPA analysis on genes that were significantly differentially expressed between sexes in GF and Conv mice (p ≤ 0.01). The top 25 regulated biological functions in GF and Conv mice based on male-female differences are shown. Hatched bars = biological functions that occur in the top 25 of both GF and Conv mice. Filled bars = biological functions that are specific for either GF or Conv mice.
