Figure 2

Tissue and Al-inducible expression pattern of GmGRPL by qRT-PCR. The soybean GmActin gene was used as the internal reference gene. (a) Tissue expression pattern of GmGRPL in root, stem, leaf, flower and seed. The different capital letters represent significant differences between tissues by ANOVA (p < 0.01). (b) Al-inducible expression pattern of GmGRPL. The soybean seedlings in the Vc stage (unifoliolate leaf fully developed) were transferred to AlCl3 (20, 50, 100, 150 μM) for 0, 2, 4, 8, 12, 24, 36, 48 h, 3 d, 4 d, 5 d and 6 d. The roots were sampled to analyse the Al-inducible expression pattern. The different capital letters represent significant differences during different times of one treatment by ANOVA (p < 0.01). The error bars represent the SEM. n = 3.