Figure 5
From: Impaired microcirculation after subarachnoid hemorrhage in an in vivo animal model
Vasoconstriction and thrombosis in the rat brain 24 hours after subarachnoid hemorrhage (SAH). Rat brain tissues were immunofluorescently stained with α-smooth muscle actin (α-SMA) for arterioles. Capillaries and thrombi inside capillaries were labeled with glucose transporter 1 (GLUT1, red) and fibrin (green), respectively. (A) Cortical arterioles were constricted in the SAH group as compared with the control group. (B) Fibrin (+) thrombi were detected in the capillaries of the brain parenchyma in the SAH group, but were absent in the control group. (C) The proportion of thrombosed capillaries (yellow) was demonstrated by double-labeling immunofluorescence of fibrin (green) and GLUT1 (red). (D,E) The arteriolar diameter and the proportion of fibrin (+) capillaries were quantitatively analyzed. Bar = 50 μm.
