Figure 9
From: Impaired microcirculation after subarachnoid hemorrhage in an in vivo animal model
Pathological examination of rat brains 24 hours after superfusion of cerebrospinal fluid from human patients (hCSF) with subarachnoid hemorrhage (SAH). Rat brains were dissected and processed for paraffin-embedding. Sections were then stained with H&E and subjected to immunohistochemical analysis. (A) Pathological examination showed that hCSF from SAH patients induced thrombosis on the affected rat brain. Thrombus formation within the affected subcortical area (arrowhead) and cortical vessels (black arrow) was demonstrated by H&E staining. (B) Positive staining of fibrin confirmed the formation of thrombi inside the vessels of the cortical (black arrow) and subcortical (arrowhead) regions. (C) Vasoconstriction of the cortical arterioles (black arrow) was clearly observed by immunohistochemical staining of α-smooth muscle actin.
