Figure 4
From: High-Reynolds Microfluidic Sorting of Large Yeast Populations
Proteomics analysis of the young and adult yeast cells (A) Volcano plot shows the (−log10) Welch t-Test P-value of each protein versus the Welch t-Test difference (log 2) between the two samples. Proteins that changed significantly (FDR = 0.05, S0 = 0.1) by at least two-folds with p-value < 0.05 are colored in blue (upregulated in young cells) or red (upregulated in adult cells). The complete list of the significantly different proteins can be found in Table S4. (B) Annotation of significantly different proteins and their enrichment over yeast proteome51, showing differences in protein folding and translation. (C) Quantitative western blot validation of selected proteins. SPB1 is equally abundant in both populations and its quantity did not change significantly (p = 0.44, n = 3). EGD1 was enriched by 2-folds in the adult population (p = 0.02, n = 3), while KAP95 was enriched by 2-folds in the young population (p = 0.049, n = 3). ×0.5 notes 2-fold dilution of ×1. (D) Interactions of significantly different subset of proteins between young and adult cell cultures. Interaction map of upregulated proteins in young culture (blue) and adult cells (red) was derived by using STRING database of interactions and visualized by Cytoscape. To reduce complexity, subnetworks were selected for presentation. On right, subnetworks of proteins upregulated in young cells interacting with chaperones CCT3 and SBA1, and mRNA associated proteins DHH1, SSD1. Central panel presents subnetwork of translation regulators in young cells.
