Figure 4 | Scientific Reports

Figure 4

From: Implications for the impairment of the rapid channel closing of Proteomonas sulcata anion channelrhodopsin 1 at high Cl− concentrations

Figure 4

The Cl− dependence on the absorption spectra of the P1–P4 states (from the top to the bottom) in the presence of (A–D) 0.1–10 mM (red, orange and green, respectively) and (E–H) 10–1,000 mM Cl− (green, blue and purple, respectively). The spectrum of P0 (grey broken line) represents pure retinal spectra. The arrows indicate the direction of the changes in absorption and accumulation. For better understanding, the assignment and the equilibrium state of the photo-intermediates are shown in each panel. The Cl− dependent equilibrium shift is indicated by the red arrow. (I–L) The photo-intermediates in the P1–P4 states are illustrated in the photocycle scheme of PsuACR1. The Cl− conducting and non-conducting states are highlighted in red and blue, respectively.

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