Figure 4
From: A soy protein Lunasin can ameliorate amyloid-beta 42 mediated neurodegeneration in Drosophila eye
Lunasin neuroprotective function is independent of retinal differentiation gene machinery, Dpp and Wg signaling pathways. (A–C) Wild-type expression of (A) Eyeless (Ey), (B) Eyes absent (Eya) and (C) Dachshund (Dac), the members of RD gene machinery in developing third instar eye imaginal discs. (A) Ey expression is anterior to MF in the third instar eye discs. (B) Eya is expressed in the differentiating photoreceptors and anterior to MF. (C) Dac is expressed along the MF as well as in antennal region. (D) Ey, (E) Eya and (F) Dac expression is not affected in the GMR > Aβ42 + Lun background. (G–I) In the developing eye imaginal discs, study of dpp expression using dpp-lacZ reporter in the (G) Wild-type, (H) GMR > Aβ42, (I) GMR > Aβ42 + Lun. Note that dpp-lacZ marks the morphogenetic furrow (MF) in the developing eye. (J–L) In the developing eye imaginal discs, study of Wg expression using a wg-lacZ reporter in the (J) Wild-type, (K) GMR > Aβ42, (L) GMR > Aβ42 + Lun. The wg is expressed on the antero-lateral margin of the developing third instar eye imaginal discs. Note that the Lun neuroprotection function is independent of Wg and Dpp signaling. Magnification of all eye discs is 20X.
