Figure 2
DCA inhibits PDK, decreases extracellular lactate and increases intracellular ROS levels in PTJ64i and PTJ86i cell lines. (A) Western blot analysis of p-PDH-E1α (pSer300) and PDH-E1α proteins after incubation of PGL cells for 24 hours with vehicle or DCA at the indicated concentrations; (B) Histograms represent quantification of extracellular lactate levels in the culture media of PGL cell lines treated with DCA or vehicle (control) for 24 hours. Data shown are the means ± SD of three replicates and are expressed as fold change relative to vehicle (control) (**p < 0.01; ***p < 0.001); (C) Intracellular ROS levels in PTJ64i and PTJ86i cells measured by flow cytometry after a 24–hour treatment with DCA or vehicle (control). Data shown are the means ± SD of three independent experiments (*p < 0.05); (D) Representative CM–H2DCFDA histograms derived from flow cytometry analysis showing ROS production in PTJ64i and PTJ86i cells after a 24–hour treatment with DCA or vehicle (control). Full–length western blots are included in Supplementary Information (Fig. S1).
