Figure 3
DCA affects cell cycle in PTJ64i and PTJ86i cell lines. (A) The histograms show the mean percentages of cells (values inside the bars) in the different cell phases, evaluated by flow cytometry, after 24-hour treatment with 12.5 or 50 mM DCA as compared to control. Results shown are the means ± SD of three independent experiments (**p < 0.01; ***p < 0.001); (B) Representative DNA distribution histograms of PGL cells exposed to DCA (12.5 mM and 50 mM) or vehicle (control) for 24 hours, as measured by flow cytometry; (C) Expression of cyclin D3 and cyclin B1 proteins after incubation of cells for 24 hours with vehicle or 50 mM DCA was analyzed by western blot using antibodies directed against the indicated proteins. Full–length western blots are included in Supplementary Information (Fig. S2).
