Figure 7
Metformin affects cell viability in PTJ64i and PTJ86i cell lines. Cells were incubated for 72 hours with metformin at the indicated concentrations, or with PBS vehicle (control). Cell viability was assessed by MTT assay. Metformin significantly inhibited cell viability in both cell lines in a dose-dependent manner, with an IC50 of 21.6 mM in PTJ64i and 22.1 mM in PTJ86i, as assessed by CompuSyn. Data shown are the means ± SD of three independent experiments with quintuplicate determinations. *Statistically significant differences between control and each drug concentration (*p < 0.05; **p < 0.01; ***p < 0.001).
