Figure 4 | Scientific Reports

Figure 4

From: Low-dose calcipotriol can elicit wound closure, anti-microbial, and anti-neoplastic effects in epidermolysis bullosa keratinocytes

Figure 4

Secretome of calcipotriol-treated RDEB cells exhibit enhanced antimicrobial activity. (a) C. albicans or P. aeruginosa (35,000 CFU early log-phase) were treated with different concentration of LL-37 (0–10 µg/ml) and the number of metabolically active cells was measured in a resazurin-based antimicrobial activity assay. No antiproliferative effect of 100 nM calcipotriol itself was observed when incubated with C. albicans (right panel) (b) NHK, RDEB-2 and RDEB-3 cells were treated with 100 nM calcipotriol (+) or vehicle control DMSO (−) for 48 hrs, and culture supernatants were harvested and used in a resazurin assay against C. albicans as described above. Mean ± SD of five experiments is shown. Statistical analysis was performed as described in the materials and methods section. (c) Dot blot assay measuring cathelicidin in concentrated cell culture supernatants of calcipotriol- vs vehicle-treated NHK, RDEB-2 and RDEB-3 cells. Decreasing amounts of spotted LL-37 peptide served as quality control.

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