Table 1 Particle sizes determined from TEM after growth of the rhodamine B labelled silica layer and growth of the second stabilization layer and corresponding fluorophore labelling efficiencies and average separations between fluorophores.
[Dye] | Total particle diameter | Labelling efficiency (%) | Average separation (nm) | Fluorophores per particle (a.u.) | |||
|---|---|---|---|---|---|---|---|
RITC layer | Stab. layer | ||||||
d (nm) | σ (nm) | d (nm) | σ (nm) | ||||
30 | 79 | 4.5 | 89 | 4.9 | 3.7 | 7.7 | 558 ± 24 |
25 | 86 | 5.4 | 88 | 4.8 | 4.1 | 7.9 | 662 ± 26 |
20 | 83 | 5.0 | 88 | 5.4 | 4.5 | 8.2 | 530 ± 23 |
15 | 82 | 4.3 | 88 | 4.8 | 4.3 | 9.2 | 358 ± 19 |
12.5 | 82 | 4.3 | 87 | 5.0 | 3.8 | 10.2 | 265 ± 16 |
10 | 82 | 4.7 | 89 | 4.5 | 3.2 | 11.7 | 175 ± 13 |
7.5 | 83 | 4.8 | 89 | 4.7 | 3.5 | 12.4 | 151 ± 12 |
5 | 81 | 4.7 | 88 | 5.1 | 2.9 | 15.2 | 77 ± 9 |
2.5 | 83 | 5.6 | 89 | 5.3 | 2.7 | 19.6 | 39 ± 6 |
1 | 82 | 4.8 | 89 | 4.5 | 2.2 | 28.5 | 12 ± 4 |
0 | 76 | 4.8 | 89 | 5.4 | N/A | N/A | N/A |
