Figure 2 | Scientific Reports

Figure 2

From: A multi-cellular 3D bioprinting approach for vascularized heart tissue engineering based on HUVECs and iPSC-derived cardiomyocytes

Figure 2

iPSC cultured in 3D scaffolds. (a) Representative images of iPSC morphology grown in bulk and iPSC 3D printed at day 0, 7 and 14. Scale bars represent 50 μm and 100 μm, respectively. (b) QRT-PCR analysis for stemness genes, such as oct4, sox2 and nanog, in iPSC cultured on MEF feeder layer, bulk hydrogel and 3D bioprinted scaffold at day 0, 7 and 14. Error bars represent mean ± SEM. Student’s t test did not evidence significant differences in gene expression among the different tested conditions. N = 4. (c) Colony size gradient of iPSC cultured in bulk and 3D bioprinted at day 14. Scale bars represent 1 mm. (d) Live/dead assay graph relative to bulk and 3D bioprinted-iPSCs after 14 days of culture.

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