Figure 2

AGEs increased HIF-1α and PDK4 expression. HIF-1α and PDK4 expression in calcified VSMCs treated with AGE-BSA at different concentrations and times were evaluated by western blotting (A,B) and qRT-PCR (C,D). *P < 0.05 compared with the normal control group. &P < 0.05 compared with the AGE-BSA (400 μg/ml) group. #P < 0.05 compared with the AGE-BSA (24 h) group.