Figure 1
From: Platelet activation and prothrombotic properties in a mouse model of peritoneal sepsis
Characterization of sepsis after cecal ligation and puncture. (a) Weight loss was increased 48 hours post procedure in the CLP group of mice (black bar) compared to the sham group (white bar). Results are expressed as percentage of weight loss and are median [25–75th percentiles] (n = 14, ***p < 0.001). (b) Survival was quantified at 72 hours post CLP. At 72 h, the overall mortality was 47% in CLP group. Results are expressed as percentage of survival (n = 36, p < 0.05). Biochemical analysis were performed with a PENTRA 400 ABXc analyzer for aspartate aminotransferase (AST) (c), alanine aminotransferase (ALT) (d) and lactate dehydrogenase (LDH) (e). Results are presented as median [25–75th percentiles] (n = 6, *p < 0.05, **p < 0.01, ***p < 0.001). (f) Leukocyte count was measured 48 h post surgery and compared in sham versus CLP group. Results are expressed as median [25–75th percentiles] (n = 6 to 30 *p < 0.05,**p > 0.001, ***p < 0.0001). (g) Representative images of lung sections stained with hematoxylin and eosin 48 h post surgery. The arrowhead shows a blood vessel section which integrity is conserved in a sham-operated mouse (a). In the CLP group of mice (b) important alveolar injuries are observed as quantified by the Acute Lung Injury (ALI) Score 48 h post CLP induction (h). Results are median ± IQR of 7 independent experiments (*p < 0.05) and representative images are shown (g).
