Figure 5
From: Platelet activation and prothrombotic properties in a mouse model of peritoneal sepsis
Platelet pro-thrombotic properties at arterial flow and bypass of PI3Kβ for thrombus stability at high shear rate during sepsis. (a) DIOC6-labeled platelets in whole blood from the CLP (black bar) or sham (white bar) mice 48 h post intervention were perfused through a collagen-coated microcapillary at a physiological arterial rate of 1500 s−1. Surface coverage (%) by fluorescent platelets was analyzed using ImageJ software. Results shown are median ± IQR of 4 independent experiments (*p < 0.05). (b) Platelet-rich thrombi formed in the liver 48 h post CLP were detected as in Fig. 2 (C-D) and quantified. 5 mice from each group and 5 to 10 field per mice were analyzed. Results are expressed as median ± IQR (***p < 0.001). (c–e) DIOC6-labeled platelets in whole blood from platelet PI3Kβ-deficient mice (p110βnull) or wild type mice (WT) were perfused through a collagen-coated microcapillary at a physiological arterial shear rate of 1500 s−1, followed by a high shear rate of 4000 s−1. Thrombi volumes (μm3) were analyzed using ImageJ software. Results are expressed as median ± IQR of 4 to 6 independent experiments (***p < 0.001). (e) Representative images showing the platelet thrombi remaining after 1 min of high shear rate (4000 s−1).
