Figure 4
From: Microfabricated tuneable and transferable porous PDMS membranes for Organs-on-Chips
The architecture used in the experiments of HUVECs culturing on transferred PDMS membranes. (a) Schematic representation of the OOC. A four-layered sandwich consisting of bottom layer with a defined microchannel (500 μm width, 325 μm height, 7 mm in length), porous PDMS membrane with defined pores (8 μm diameter, 4 μm in thickness), top layer with the same channel dimensions as the bottom layer, and PDMS slab with defined reservoirs for media refreshing (5 mm in diameter). (b) Close-up of the microchannel area with transferred membrane of 4 μm in pore size. (c) Close-up of the microchannel area with transferred membrane of 8 μm in pore size. Scale bars: 100 μm.
