Figure 6

MDA cell transmigration through porous PDMS membrane transferred to a OOC. (a) Schematic of the OOC used for EGF driven migration, showing the inlets for top and bottom microchannel (400 μm width, 100 μm height) where EGF is added. Cells were seeded at the bottom side of the membrane, serum starved overnight and exposed to a gradient of EGF towards the top channel, separated with either a (b) nonporous; (c) 2.0 ± 0.3 μm pore size (Type A); (d) 3.0 ± 0.3 μm pore size (Type B) and (e) 10.0 ± 0.3 μm pore size (Type C) membrane. Arrows indicate cells (e) or cellular processes (c,d) probing in the channels. Scale bar: 50 μm.