Figure 4 | Scientific Reports

Figure 4

From: Antiviral innate immune response in non-myeloid cells is augmented by chloride ions via an increase in intracellular hypochlorous acid levels

Figure 4

Viability of different non-myeloid cells in the presence of sodium chloride: Viability of A549 (a), 3T3 (b), HuH-7.5 (c), and HuH-7 (d) cells were not significantly impaired in the presence of NaCl. Cells were treated with increasing concentrations of NaCl (in triplicate). 48 hours post-treatment cellular viability was determined with CellTiter-Blue (Promega) by the ability of cells to metabolise the substrate to produce a fluorescent end-product. Cell viability was normalised to untreated cells (0 mM NaCl). Viability below 70% is evidence of cytotoxicity. While A549 and 3T3 cells were viable up to 100 mM NaCl, HuH-7.5 and HuH-7 cells were viable only up to 50 mM NaCl. Error bars represent the standard error of the mean of 3 independent experiments carried out in triplicate. NaCl (mM) values are over and above that found in DMEM (110 mM).

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