Figure 7
HSV-1 is inhibited in the presence of NaCl by increased production of intracellular hypochlorous acid: HeLa cells were infected with non-GFP labelled HSV-1 at MOI 0.5 for 1 hour before treating with increasing concentrations of NaCl. After 6 hours, cells were washed and stained with 10 μM HCSe8 (a BODIPY-based green fluorescent probe which is rapidly and specifically oxidized by HOCl to emit a fluorescence signal) or R-19S (a rhodamine fluorophore)9 for 30 minutes. Fluorescence was measured after cells were washed twice with PBS. Fluorescence values were normalized to uninfected cells treated with the corresponding NaCl concentration. NaCl (mM) values are over and above that found in DMEM (110 mM). Error bars represent the standard error of the mean of three biological replicates. *p < 0.05 and **p < 0.01 when compared to 0 mM NaCl.
