Figure 1
From: Anti-TNFR1 targeting in humanized mice ameliorates disease in a model of multiple sclerosis
Treatment of EAE with ATROSAB reduces disease severity. (A) Wild type C57BL/6 J mice (n = 6) and hu/m TNFR1ki (n = 5) mice were both immunized with MOG35−55 and the course of EAE followed until 28 days after disease onset. No difference was seen between the courses of EAE in the two strains of mice. (B) Weight loss was also assessed, again revealing no differences between the mouse strains. (C) hu/m TNFR1ki mice were treated by intra-peritoneal injection with either 20 mg/kg ATROSAB (n = 6) or a corresponding control IgG (n = 5) on days 1, 4, 8 and 12 of manifest EAE and followed until day 28 of EAE. ATROSAB treatment led to a significant reduction in disease severity from the third day of EAE onwards. (D) Assessment of EAE-associated weight loss similarly revealed a significant improvement in the mice receiving 20 mg/kg ATROSAB. (E) Analysis of individual EAE scores of ATROSAB-treated mice from the experiment shown in panels C/D. Two mice (green diamonds) out of 6 appear to stop responding to treatment by the end of the experiment at day 28 of EAE. (F) Blood sera samples from these ATROSAB-treated mice were assessed for the presence of anti-ATROSAB antibodies by ELISA, demonstrating a positive correlation between anti-drug antibody levels and disease severity at experiment end (Pearson correlation coefficient of 0.75). All EAE studies were repeated, with one representative experiment being shown. */**p < 0.05/0.01.
