Figure 5
Heme coordination. (a) Adding heme to a bis-Histidine variant of REAMP causes a colour change from green to red indicative of cofactor binding. (b) Representative UV/Vis spectroscopy showing spectral changes characteristic of heme binding to REAMPS35H/L94H. (c) Both mono-His and bis-His variants exhibited tight binding. Data are mean ± s.d, n = 3. (d) Bis-His coordination shifts the heme redox potential. Values are from fitting the reductive sweep (closed symbols) to the Nernst equation for a single electron reduction.
